3 edition of Protein synthesis in the preblastoderm embryo of Drosophila melanogaster found in the catalog.
Protein synthesis in the preblastoderm embryo of Drosophila melanogaster
Tyler B. Parr
Written in English
|Statement||by Tyler B. Parr.|
|LC Classifications||Microfilm 84/2625 (Q) |
|The Physical Object|
|Pagination||xi, 166 leaves : ill.|
|Number of Pages||166|
|LC Control Number||84249884|
Drosophila melanogaster wild-type strain Oregon R-P2 (Allis et al., ) was used throughout. Flies were raised at room temperature on a medium of cornmeal, agar, soy bean meal, malt extract, molasses, yeast, % (vol/ vol) propionic acid, and the mold inhibitor methyl p-hydroxybenzoate (Caesar and Lorentz, Hilden, Germany).Cited by: The tiovivo (tio) gene of Drosophila encodes a kinesin-related protein, KLP38B, that colocalizes with condensed chromatin during cell -type function of the tio gene product KLP38B is required for normal chromosome segregation during mitosis. Mitotic cells in tio larval brains displayed circular mitotic figures, increased ploidy, and abnormal anaphase by:
Summary. The possibility that the ribosomes of a temperature-sensitive lethal allele of suppressor of forked, l(1)su(f) ts67g, contain a mutated protein was studied by two-dimensional gel results from these analyses revealed no differences in the ribosomal proteins between the mutant and the wild-type strain was found, however, that the transition from larval to Cited by: Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo J Baker, J Baker Department of Zoology, University of Washington, Seattle Drosophila embryogenesis is initiated by a series of syncytial mitotic divisions. cell-cycle dependence, and protein synthesis requirement Cited by:
Drosophila melanogaster life cycle. The entire life cycle lasts approximately 10 days at 25°C. Flies complete embryonic development as eggs before hatching as first instar larvae. Disks large 1 tumor suppressor protein Drosophila melanogaster (Fruit fly) Stage 14 and 15 embryos exhibit expression in the developing body wall muscle. Expression in neuropil regions of the CNS and at NMJs persists through to larval development. Other isoforms show .
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An early period of pre-programmed development is not unique to the Drosophila embryo. For instance, Xenopus development has features that parallel the early stages of Drosophila embryogenesis: its early divisions are rapid, stereotyped and synchronous, and RNA synthesis is “low-to-undetectable”.Cited by: The preblastoderm embryo of Drosophila melanogaster is characterized by a series of extremely rapid nuclear divisions.
In the absence of relevant protein synthesis replication of the genomes and their concomitant assembly into chromatin relies entirely on stockpiles of maternal histones Cited by: An analysis of stage-specific protein synthesis in the early Drosophila embryo using high-resolution, two-dimensional gel electrophoresis ☆ Author links open overlay panel Michael C.
Summers 1 Vahe Bedian ∗ Stuart A. KauffmanCited by: Here, we present a comparison of the proteome of two extreme developmental states in the well-established model organism Drosophila melanogaster: adult and embryo. Protein modules such as ribosome, proteasome, tricarboxylic acid cycle, glycolysis, or oxidative phosphorylation were found differentially expressed between the two developmental by: 1.
The follicle cells are a major site of vitellogenin synthesis in Drosophila melanogaster. Developmental Biology89 (1), DOI: /(82) Carol T. Bradford, David T. Sullivan. Isolation of polysomes from larval and adult Drosophila by: We determined the absolute rates of RNA synthesis during embryogenesis in Drosophila melanogaster by measuring the incorporation of 3 Horotic acid into RNA, and the specific activity of the UTP pool.
Initially (preblastoderm) the rate of RNA synthesis is relatively high, but declines to a lower level by by: Gutzeit HO, Gehring WJ () Localized synthesis of specific proteins during oogenesis and early embryogenesis inDrosophila melanogaster.
Wilhelm Roux's Archives – Google Scholar Gutzeit HO () Expression of the zygotic genome in blastoderm stage embryos of Drosophila: analysis of a specific by: Author Summary Genetic studies identified many genes that are required during Drosophila oogenesis to endow the embryo with structures and components it will need to develop; they have also identified many genes that the embryo must express.
However, measures of transcription have detected zygotic transcripts only after seven nuclear divisions, and many studies have concluded that zygotic. Embryos at various stages of early development from to 5 hr after oviposition were made permeable with octane and labeled for 1 hr with [3H]phenyl Cited by: Optimization of a tissue-specific ribosome profiling approach in Drosophila Ribosome profiling is a powerful approach for measuring genome-wide changes in mRNA translation.
However, high quantities of starting material is necessary to obtain sufficient amounts of ribosome protected mRNA fragments for the subsequent processing steps involved [ 29 ].Cited by: DEVELOPMENTAL BIOL () Autoradiographic Study of Protein and RNA Formation during Early Development of Drosophila Eggs MARKO ZALOKAR Centre de Genetique Moleculaire de C.N.R.S., Gifsur Yvette, France Accepted Octo Permeabilized eggs of Drosophila melanogaster were incubated in tritiated uridine, valine, and by: " but our knowledge is so weak that no philosoph er will ever be able to completely explore the nature of even a fly " * Thornas Aquinas "In Syrnbolurn Apostolorum" RSV p/96 This is a monograph on embryogenesis of the fruit fly Drosophi la melanogaster conceived as a reference book on morphology of embryonie development.
Summary. Protein synthesis in egg follicles and blastoderm embryos ofDrosophila melanogaster has been studied by means of two-dimensional gel electrophoresis. Up to polypeptide spots have been resolved on autoradiographs. Stage 10 follicles (for stages see King, ) were labelled in vitro for 10 to 60 min with 35 S-methionine and cut with tungsten needles into an anterior fragment Cited by: In the Drosophila preblastoderm embryo, however, centrosomes are bound to nuclei deep within the embryo.
Nevertheless, there are nonpolymerized and short polymerized forms of tubulin localized immediately beneath the plasma membrane to a depth of 10 μm (Karr and Alberts, ).Cited by: The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis.
Of the proteins scored, 68 (26%) show dramatic changes in rates of synthesis during the first 8 h of embryogenesis. These stage-specific proteins can be classified into three categories: early, detected at 1, 2 and 3 h but not later; late, not Cited by: Abstract.
To gain a better understanding of the factors that can mediate chromatin assembly, we have purified and cloned a core histone-binding protein from Drosophila melanogaster embryos. This protein resembles Xenopus laevis nucleoplasmin, and it has therefore been termed dNLP, for Drosophila nucleoplasmin-like protein.
dNLP is a nuclear protein that is present. Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo. Baker J(1), Theurkauf WE, Schubiger G. Author information: (1)Department of Zoology, University of Washington, Seattle Drosophila embryogenesis is initiated by a series of syncytial mitotic by: Cell performed an electron microscopic study of the morphology and spatial distribution of replicons in S phase chromatin of cellular blastoderm D.
melanogaster embryos. Drosophila embryos are particularly useful for such a by: Protein synthesis in all cells is carried out by macromolecular machines called ribosomes.
Although the structures of prokaryotic, yeast and Cited by: The fruit fly, Drosophila melanogaster (Meigen, ) has been established as a cornerstone for research into a wide array of subjects including diseases, development, physiology, and genetics. Thanks to an abundance of genetic tools, publicly available fly stocks, and databases, as well as their considerable biological similarity to mammalian systems, Drosophila has been solidified as a key Author: Mariateresa Allocca, Sheri Zola, Paola Bellosta.
Mitochondria are inherited maternally as globular and immature organelles in metazoan embryos. We have used the Drosophila blastoderm embryo to characterize their morphology, distribution and Cited by: 7.Protein synthesis has been investigated in cell-free preparations from mature ovarian oocytes, unfertilized and fertilized eggs, and early embryos of Drosophila melanogaster.
Preparations from unfertilized eggs have a specific activity that is 5- to 6-fold higher than the activity of fractions from ovarian oocytes.
There is an additional small Cited by: Monoclonal antibodies were prepared against a fraction of nuclear proteins of Drosophila melanogaster identified as tightly binding to DNA.
Four of these antibodies were directed against a kilodalton nuclear protein; immunofluorescence staining of the polytene chromosomes localized the antigen to the alpha, beta, and intercalary heterochromatic by: